Association of multiple SNPs at slc2a9 exon 8 in gout disease in Iraqi population: A molecular study

This work was planned to reveal changes in DNA sequence coding for GLUT9 by SLC2A9 gene at exon 8 that may be play a role in gout disease. For this reason 250 male and 150 female patients were involved distributed as follow: patients with gout included 110 male and 15 female, patients with gout and diabetes (T2DM) included 135 male and 90 female, and patients with diabetes and hyperuricemia were 35 male and 15 female and 150 healthy subject served as control. DNA sequence analysis of acquired from each group showed presence of one SNP and DNA deletion in patients with gout disease, five SNPs in patients with gout and T2DM, and one SNP accompanied with rs734553 in patients showing hyperurecemia and T2DM. Translation and alignment of these sequences with control showed significant change in amino acid sequence in all patient groups investigated. DNA polymorphism, codon usage bias, and linkage disequilibrium showed significant association of SNPs detected with gout, and common SNP at position 24,706 of the gene may be with T2DM. This SNP was sent for registration at NCBI under submission ID MW2438870.