Biochemical and molecular study of long non-coding RNAs (HOTTIP, ZEB-AS1 and MEG-3) in hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is a main cause of cancer death worldwide. The high mortality of HCC patients is linked to delayed diagnosis. Long non-coding RNAs (lncRNAs) have been demonstrated as significant controllers of various human cancers. This study aimed to clarify the potential diagnostic and prognostic value of lncRNAs (HOTTIP, ZEB-AS1 and MEG3) in HCC. This study included 150 subjects (50 HCC patients, 50 cirrhotic patients and 50 healthy subjects). lncRNA gene expression was estimated by real-time PCR. HOTTIP and ZEB-AS1 levels were higher in HCC patients than in cirrhotic patients and healthy individuals (P 15 and