Quantitative detection of tapered fiber SERS probes prepared in batches by electrostatic adsorption self-assembly method

•Developing a batch fabrication scheme of tapered fiber SERS probes with excellent interchangeability.•Proposing and demonstrating a spectral data assimilated method.•Obtaining a high-precision calibrated SERS quantitative relationship curve.•Achieving excellent test recovery rate in SERS quantitative detection. The tapered fiber Surface-enhanced Raman scattering (SERS) probe is a highly sensitive SERS substrate for on-site detection. However, fiber probes prepared by the current technology lack interchangeability and are difficult to meet the practical quantitative detection demands. In this paper, we develop a batch preparation technology of tapered fiber SERS probes, with good interchangeability between each of the 20 tapered fiber SERS probes prepared in batches. Furthermore, we propose and demonstrate a spectral data assimilation method, through which the differences in interchangeability between different batches can be eliminated. Using 10 batches of spectral data assimilated with this method, we obtain a high-precision calibrated quantitative relationship curve with a fitting degree of 0.99464, with recovery rates of the two thiram spiked samples 106.88% and 95.75%, respectively. The batch preparation technology and the spectral data processing method provide a new option for practical quantitative detection.