Smart quick response code for multianalyte determination

The colorimetric determination of glucose in a sample is carried out by glucose oxidase enzyme which catalyzes the oxidation of glucose to gluconic acid and hydrogen peroxide in presence of atmospheric oxygen. Alike, the determination of lactate is carried out by lactate oxidase enzyme which catalyzes the oxidation of lactate to pyruvate and hydrogen peroxide in presence of atmospheric oxygen. The formed hydrogen peroxide reacts under catalysis of peroxidase (HRP) with reduced TMB which changes its color to blue (TMBox) acting as indicator. [Display omitted] •Analytical QR code.•Multianalyte information.•Smartphone positioning correction.•Ambient conditions correction. The extensive use of smartphones with point-of-care (POC) applications has boosted the development of what is commonly known as Healthcare 4.0. The ability to track biomarker parameters has become a critical part of monitoring the progression of certain diseases, with a potential application in a large population group. In this line, this work presents a multianalyte quick response (QR) colorimetric code for real-time measurement of glucose and lactate samples using smartphones. The colorimetric QR code contains both QR static data and dynamic information from sample analyses. Two correction methods are applied in order to compensate for the variation in the surrounding conditions during biomarker tracking. Glucose and lactate recovery between 91 and 117% was found in assays including sweat and serum samples through smartphone readout.