Direct electrochemistry of hemoglobin/peptide-carbon nanotube modified electrode for hydrogen peroxide biosensing
•GCE modification with peptide-SWNTs dispersions, enhances its electrochemical response.•This modification provides simple and efficient hemoglobin immobilization for H2O2 detection.•The modified electrode was sensitive to different hydrogen peroxide concentrations. Mediatorless enzymatic biosensors...
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Veröffentlicht in: | Materials letters 2023-03, Vol.335, p.133799, Article 133799 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | •GCE modification with peptide-SWNTs dispersions, enhances its electrochemical response.•This modification provides simple and efficient hemoglobin immobilization for H2O2 detection.•The modified electrode was sensitive to different hydrogen peroxide concentrations.
Mediatorless enzymatic biosensors based on direct electron transfer show promise due to their high stability and remarkable electrocatalytical performance. In this study, the direct electrochemistry of a glassy carbon electrode modified with hemoglobin (Hb) embedded in a hybrid layer of self-assembling EFK8 peptide and single-walled carbon nanotubes (SWNTs) was investigated. Electrochemical studies in 0.1 M phosphate buffer (PB) revealed that the immobilized Hb did not denature and retained its bio-catalytic activity. The resulting biosensor was used to detect and measure the hydrogen peroxide (H2O2) concentration in 0.1 M PB (pH 7.0), yielding a linear detection range from 20 to 960 µM and a detection limit of 7.54 µM (S/N = 3). The EFK8-SWNT hybrid layer shows promise as a biocompatible layer for simple non-covalent enzyme immobilization as the basis for future mediatorless enzymatic biosensors. |
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ISSN: | 0167-577X 1873-4979 |
DOI: | 10.1016/j.matlet.2022.133799 |