Risk investigation and diversity of microbial contamination during slaughter processing of yellow-feathered broiler

The cleanliness of slaughter process is very pivotal to ensure the safety of broiler products, and slaughter process is prone to cause microbial contamination since it involves a highly collaborative system including different operations. The procedures in slaughter processing are primary sources of bacterial contamination, and it is crucial for poultry industry to maximize product safety and quality by understanding the connection between bacterial diversity on chicken carcasses. The commercial yellow-feathered broiler plant was investigated to analysis the risk of microbial contamination. In this study, 16S rRNA sequencing was used to evaluate microbial diversity and microbial contamination was investigated by collecting 910 samples from different processing steps. Generally, total count of bacteria colonies ranged from 3.14 log CFU/cm2 to 5.68 log CFU/cm2, and the microbial load at yellow-feathered broiler carcass samples was significantly higher than that at environmental samples; moreover, the cutting areas were the high-risk areas of cross-contamination. In addition, the relative abundance of Actinobacteria (9.61%) at water samples was significantly higher than that at contact surfaces samples. This paper provides a valuable theoretical insight into the diversity and changes in the bacterial community during slaughter beneficial for enterprise managers further enhancing yellow-feathered chicken quality. •Diversity of microbial contamination excavated three contamination sources.•The 16S sequencing showed there was cross contamination in different operating regions.•The total count of bacteria colonies ranged from 3.14 log CFU/cm2 to 5.68 log CFU/cm2.•The cutting areas were the high-risk areas of cross-contamination.•Actinobacteria at water samples was significantly higher than that at contact surfaces samples.