The effect of Ag substitution on physico-chemical and biological properties of sol-gel derived 60%SiO2-31%CaO-4%P2O5-5%TiO2 (mol%) quaternary bioactive glass

1The T/A BGs were synthesized by addition of TiO2 and Ag2O.2The hydroxyapatite globules were formed after 7 days of immersion in SBF solution.3The structure and morphology of specimens were characterized.4Cells biological responses and antibacterial activity of specimens were studied.5TA2 BG was the optimum specimen for using in clinical applications. This research presents an investigation on the simultaneous effect of titanium (Ti) and silver (Ag) on structural and biological behaviors of the co-substituted 58S bioactive glass. Structural and morphological characterizations were studied by means of X-ray diffraction (XRD) analysis, Fourier transform infrared spectrum (FTIR), scanning electron microscopy (SEM) as well as Energy-dispersive X-ray Spectroscopy (EDS) analysis. The structural results exhibited that the hydroxyapatite)HA) layer was formed on the T/A-BGs after the 7th day of immersion in simulated body fluid)SBF(solution and increased overtime. Furthermore, the trend of cell proliferation and antibacterial activity were the same and by increasing Ag percentage up to 8 mol.% firstly, mentioned properties increased and then decreased. Hence, T/A-BG containing fixed 5 mol.% Ti and 2 mol. % Ag (TA2 (indicated higher in vitro biological performance compared to other synthesized T/A-BGs and suggested as an optimal specimen for further clinical applications. [Display omitted]