Detection of methamphetamine using aptamer-based biosensor chip and cyclic voltammetry technique

In this study, polycarbonate coated with Au nanoparticles and anti-methamphetamine aptamer is used for the detection of methamphetamine. Immobilization of aptamer on Au nanoparticle modified polycarbonate surface, and monitoring of methamphetamine were evaluated with AFM, ATR-FTIR, and DRS techniques. FT-IR reflection spectrum shows a pick at 3300–3500 ​nm range (related to amine groups of aptamer bases). After immobilization of aptamer on Au nanoparticle modified polycarbonate, 8% decrease in reflection was observed. To evaluate of detection power of the designed nanobiosensor, its sensitivity for the detection of different concentrations of methamphetamine was measured with cyclic voltammetry. The detection limits of the designed nanobiosensor in two methods of FT-IR and cyclic voltammetry were 2 and 5 ​μM, respectively. Notably, the designed nanobiosensor in two different methods didn't show any response to glucose. [Display omitted] •Detection of methamphetamine at low concentration.•The designed biosensor does not response to glucose.•It could be used in real samples.