Phytotoxicity test in check: Proposition of methodology for comparison of different method adaptations usually used worldwide

Seed germination tests have been widely used in recent years to indicate the toxicity levels of samples of organic compounds, biosolids, residues and effluents. Lactuca sativa L, commonly known as lettuce, has been one of the main indicative species for these tests due to its high sensitivity to low levels of toxicity, when compared to other seeds and also because it is cultivated worldwide. Although this type of analysis or essay is being widely used, it is necessary to reflect on the various adaptations of methods used by different researchers worldwide. This work presents an innovative methodology that makes it possible to compare the different phytotoxicity methods currently used in the world, through four stages that include the coefficient of variation (CV) as the main classification criterion, also counting on an eliminatory criterion. The existence of a significant difference (P value