Gelatin solution pH and incubation time influences the size of the nanoparticles engineered by desolvation

Gelatin nanoparticles (GNPs) have been extensively engineered by desolvation processes. However, engineering monodisperse GNPs of reproducible size remains a challenge. Therefore, the starting material processing steps such as gelatin solution pH adjustment and incubation time prior to desolvation were investigated to understand their implications on GNPs size and size distribution. The GNPs were engineered by one-step and two-step desolvation processes using ethanol as a desolvating agent. Irrespective of the desolvation steps, gelatin solution pH incubation times between 0 h and 24 h showed significant (p