Sustainable Myco-Synthesis of antimony oxide nanoparticles using endophytic Penicillium chrysogenum Extract: Characterization, antimicrobial Potency, and cytotoxicity assays

[Display omitted] •Green biofabrication antimony oxide nanoparticles via the endophytic Penicillium chrysogenum ethyl acetate (PCE) extract.•Pure monoclinic crystallographic system of with an average crystallite size of 20.2 nmwere achieved.•As-prepared antimony oxide nanoparticles have a good antibacterial role. Nanobiotechnology is a grow-fast applied science that has established straight forward shots in medicine, agriculture, and industry. Herein, a proceeding investigation on the myco-synthesis of antimony oxide nanoparticles (SbO2 NPs) using endophytic Penicillium chrysogenum ethyl acetate (PCE) extract. Biosynthesized SbO2 NPs were characterized and examined along with PCE extract for their antibacterial and cytotoxic potency, in addition to analyzing the phyto-constituents of PCE extract by high performance liquid chromatography (HPLC). The obtained results showed well characterized spherical and semi-spherical SbO2 NPs with average particle size of 22.2 nm. SbO2 NPs presented high antibacterial activity against tested bacterial strains with MIC ranging between 150 to 200 µg/mL compared to the PCE extract. PCE extract and its biofabricated SbO2 NPs exhibited weak cytotoxicity against MCF-7 cell line. HPLC analysis revealed the majority of ferulic, cinnamic, rosmarinic and gallic acids in PCE extract. Fungal extracts and their biosynthesized nanoparticles with diverse bioactivities are considered prospective horizons in biotechnological applications.