Fabrication and characterization of starch/agarose biopolymers containing graphene oxide towards the release of 5-fluorouracil in cancer treatment

[Display omitted] •Focused on conveyance is attainable to tumor destinations with a lower pH than typical cells.•Biocompatible materials were used in the union of pH-responsive nanocarriers.•The amalgamation of nanoparticles was conducted utilizing the W/O/W twofold emulsion strategy.•Cytotoxicity and cellular apoptosis uncovered more prominent viability than the free drug.•This research with the conclusion of FTIR/XRD/DLS/zera/MTT showed more fruitful results than the previous research. The use of drug nanocarriers with effective drug loading can be effective in cancer treatment. In this study, graphene oxide (GO)/agarose (Aga)/starch (S) nanocarriers were made to deliver the 5-Fluorouracil (5-FU) as an anti-cancer drug to suppress breast cancer cells. The X-ray-diffraction analysis (XRD), Fourier-transform-infrared spectroscopy (FTIR), and water-in-oil (w/o) emulsification methods, zeta potential, and Dynamic-Light-Scattering (DLS) were employed for analysis. The results show that all materials are homogeneously placed next to each other. The efficiency of drug entrapment and loading in this nanocarrier was obtained as 87.25% and 46.5%, respectively, which is a suitable and significant amount. The kinetics and release of the drug show that the synthesized nanocarrier is sensitive to pH, and its release is stable. Flow cytometry outcomes showed apoptosis in cancer cells. This research’s outcomes are potent for the development and expansion of targeted drug delivery and medicinal nanomaterials based on GO/Aga/S.