A combined multi-pathlength and wavelength optimization method for accurate detection of platelet count
•First of all, high quality spectral data are obtained through the design of experimental scheme.•In order to increase the information of spectral data, the absorption spectra of three optical paths are combined.•Wavelength optimization method is used to reduce wavelength redundancy caused by spectr...
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Veröffentlicht in: | Infrared physics & technology 2022-06, Vol.123, p.104174, Article 104174 |
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Sprache: | eng |
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Zusammenfassung: | •First of all, high quality spectral data are obtained through the design of experimental scheme.•In order to increase the information of spectral data, the absorption spectra of three optical paths are combined.•Wavelength optimization method is used to reduce wavelength redundancy caused by spectral combination.•Through the combination of multi-pathlength and wavelength optimization, the detection accuracy of platelet count is improved.
Spectrometry has progressed in the rapid detection of blood components, but due to their complexity, meeting clinical detection standards remains challenging. To improve the accuracy of spectroscopic detection of blood components, this paper collected the spectra of 390 blood samples at three positions in a cuvette, and calculated the absorption spectra of three different optical path lengths. Then, using platelet count as the target component, the wavelength was optimized by the PLS least square (PLS) method. The experimental results show that the multi-pathlength joint modeling outperformed the single-pathlength modeling, and the detection accuracy is further improved after the multi-pathlength spectrum is processed by wavelength optimization. The correlation coefficients of calibration and prediction are 0.925 and 0.901, and the root mean square errors (RMSE) are 23.43 and 25.28, respectively for the two sets. The experiment shows that the combination of multi-pathlength modeling and wavelength optimization can provide a new reference for improving the accuracy of complex liquid solution components by spectroscopy. |
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ISSN: | 1350-4495 1879-0275 |
DOI: | 10.1016/j.infrared.2022.104174 |