Purification of bioactive compounds from rosemary by-products for innovative solutions in food industry

Maximizing the value of agricultural by-products is critical for advancing sustainable development and minimizing waste. This study delves into the solid by-products of Rosmarinus tournefortii de Noé, focusing on their fractionation and purification to unveil valuable bioactive compounds. Using flas...

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Veröffentlicht in:Industrial crops and products 2025-01, Vol.223, p.120125, Article 120125
Hauptverfasser: Ziani, Imane, Bouakline, Hamza, Merzouki, Mohammed, Fauconnier, Marie‑Laure, Sher, Farooq, Bentouhami, Nour Eddine, Asehraou, Abdeslam, El Bachiri, Ali
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Sprache:eng
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Zusammenfassung:Maximizing the value of agricultural by-products is critical for advancing sustainable development and minimizing waste. This study delves into the solid by-products of Rosmarinus tournefortii de Noé, focusing on their fractionation and purification to unveil valuable bioactive compounds. Using flash chromatography, nine distinct fractions (F1 to F9) were successfully isolated. Cutting-edge analytical techniques including FTIR, UHPLC-MS/MS, and 2D NMR (HSQC, HMBC and COSY) confirmed the presence of novel labdane diterpenoids (PF1), 24-nor-ursane triterpenoids (PF5), and ent-kaurane diterpenoids (PF6). Furthermore, HPLC-DAD profiling revealed rosmadial (79.43 % in F4) and luteolin (70.14 % in F7) as the dominant phenolic compounds. Notably, the crude extract demonstrated remarkable antioxidant activity, with an IC50 of 0.04±0.23 mg/mL. Fractions F7 and F8 also exhibited strong antioxidant potential, with IC50 values of 0.35±0.07 mg/mL and 0.36±0.02 mg/mL, respectively. Purified fractions PF7 and F8, enriched with luteolin and 3-hydroxyflavone, proved highly effective for pigmentation control, with PF7 showing an IC50 of 0.045±0.007 mg/mL. In addition, antimicrobial assays revealed that the 24-nor-ursane triterpenoid and fraction F6, containing ent-kaurane and carnosol, displayed potent inhibitory effects against Rhodotorula glutinis (24.1 mm). Molecular docking studies further highlighted PF5 as a potent inhibitor of alpha-amylase (-5.856 kcal/mol) and tyrosinase (-4.385 kcal/mol), while PF1 surpassed acarbose in alpha-glucosidase inhibition with a binding energy of −5.898 kcal/mol. Collectively, these findings highlight the immense potential of R. tournefortii by-products as a rich source of bioactive compounds for health and skincare, offering promising, sustainable solutions for the pharmaceutical, cosmetic and food preservation industries. [Display omitted] •FTIR, UHPLC-MS/MS, and 2D NMR revealed novel diterpenoids and triterpenoids.•Nine distinct bioactive fractions were effectively isolated by flash chromatography.•Flavonoid and phenolic fractions showed strong antioxidant and tyrosinase inhibitors.•Triterpenoids and diterpenoids showed strong antimicrobial activity, up to 24.1 mm.•Docking confirmed triterpenoids as potent alpha-amylase and tyrosinase inhibitors.
ISSN:0926-6690
DOI:10.1016/j.indcrop.2024.120125