Exploring surface properties of substrate to understand the difference in enzymatic hydrolysis of sugarcane bagasse treated with dilute acid and sulfite

[Display omitted] •Hydrolysis yield of bagasse treated with bisulfite was higher.•More cellulase was adsorbed on bagasse treated with bisulfite.•Bagasse treated with bisulfite had smaller binding strength with cellulase.•Pore profiles of bagasse treated with bisulfite were more advantageous.•Cellulo...

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Veröffentlicht in:Industrial crops and products 2020-03, Vol.145, p.112128, Article 112128
Hauptverfasser: Lan, T.Q., Zheng, W.Q., Dong, Y.F., Jiang, Y.X., Qin, Y.Y., Yue, G.J., Zhou, H.F.
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Sprache:eng
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Zusammenfassung:[Display omitted] •Hydrolysis yield of bagasse treated with bisulfite was higher.•More cellulase was adsorbed on bagasse treated with bisulfite.•Bagasse treated with bisulfite had smaller binding strength with cellulase.•Pore profiles of bagasse treated with bisulfite were more advantageous.•Cellulose might adsorb cellulase by COR bonds. The sulfite pretreatment to overcome recalcitrance of lignocellulose (SPORL) is beneficial to subsequent enzymatic hydrolysis. In this study, to elucidate enzymatic hydrolysis mechanism of SPORL treated sugarcane bagasse (SCB), the surface properties of SPORL and dilute acid (DA) treated SCBs were compared, including cellulase adsorption isotherm and kinetics, specific surface area and average size of pores, water retention value (WRV), element composition and percentages of different chemical bonds on the substrate surface. The results showed the hydrolysis yield of SPORL SCB (76.16 %) was higher than that of DA (57.82 %) and SPORL SCB had better adsorption capacity, lower binding strength, a higher cellulase adsorption rate, larger specific surface area and higher WRV, and these two SCBs might adsorb cellulase by COR bonds (R: organic groups), suggesting SPORL SCB’s surface was more advantageous to enzymatic hydrolysis. This study could help understand the mechanism of lignocellulosic hydrolysis in depth.
ISSN:0926-6690
1872-633X
DOI:10.1016/j.indcrop.2020.112128