Rapid safety assessment of reconstituted infant formula using electrical impedance analysis

A rapid method to assess freshness is crucial for the safe handling of reconstituted infant formula (RIF) and prevention of food-borne illnesses in infants. This study explored the use of electrical impedance measurement (EIM) at a constant frequency for real-time freshness evaluation of RIF. Initial electrical impedance of 89.16 ± 0.47 Ω decreased to 74.29 ± 0.23 Ω within 48 h at room temperature (20 °C) storage. Under temperature abuse conditions (30 °C), the electrical impedance rapidly dropped from 75.48 ± 0.52 Ω to 60.15 ± 4.76 Ω within 18 h, indicating more rapid spoilage. Notable changes in the electrical impedance were observed during temperature abuse. Empirical model analysis revealed a strong positive correlation between electrical impedance and conventional freshness parameters such as pH, protein, and microbial growth. This study demonstrates the potential of EIM for the rapid detection of freshness changes in RIF, offering valuable insights into the prevention of food-borne illnesses in infants. •Electrical impedance estimates to spoilage of reconstituted infant formula (RIF).•Electrical impedance of RIF decreased with spoilage.•Instrumental analysis was successfully model fitted to electrical impedance.•Empirical model could estimate the microbial growth of RIF with impedance change.