Effectiveness of organic biocide in long-term protection of bamboo against mold fungi with Trametes versicolor pre-treatment

The long-term protection of bamboo against mold fungi remains a challenge in anti-mold technology, primarily due to the abundance of nutrients within bamboo cell lumens and the poor permeability of bamboo. This study aimed to enhance the effectiveness of organic compound biocide in the long-term protection of bamboo against mold fungi through bioincising pretreatment with Trametes versicolor. The results showed that bamboo pretreated with bioincising exhibited long-term mold resistance with an efficacy of 100 % in both 20-week laboratory and 3-month outdoor tests. During T. versicolor bioincising, the fungal hyphae invaded the interior of the bamboo, leading to a notable 56.6 % increase in biocide uptake while causing an 8 % reduction in mass loss. Furthermore, bamboo pretreated with T. versicolor exhibited a reduction in both starch and reducing sugar contents, and a slight decrease in lignin and cellulose contents. These findings suggested that the improved long-term mold resistance was primarily due to the consumption of nutrients within the cell lumen and increased absorption of organic biocide by T. versicolor bioincising. This study highlighted the use of bio-pretreatment to improve the long-term mold resistance of bamboo, thus prolonging the durability of bamboo-based products. •Improve mold resistance of bamboo by white rot fungi bioincising pretreatment.•Anti-mold efficiency of modified bamboo was 100 % in laboratory and outdoor tests.•Biocide penetration in bamboo was enhanced by T. versicolor bioincising.•T. versicolor consumed a large amount of nutrients within the cell lumen.•The breakdown of bamboo cell walls usually occurs after the depletion of nutrients.