Association of CDKAL1 gene polymorphisms with type 2 diabetes mellitus in a sample of Iraqi population

Type 2 Diabetes Mellitus (T2DM) is a multifactorial disorder in which genetic in addition to environmental factors such as excess carbohydrate intake and sedentary lifestyle play crucial role in the pathogenesis. Genome-wide association study (GWAS) identified many genes that impact on pathogenesis of T2DM, also the role of these genes in complications of diabetes and glucose homeostasis. A case-control study consisted of 400 T2DM patients and 400 control was conducted. Phenotyping and anthropometric data included body mass index (BMI), fasting plasma glucose (FPG), lipid profile and insulin levels applied for two groups. Genotyping of CDKAL1 gene single-nucleotide polymorphisms (SNPs) (rs7754840 G>C and rs7756992 A>G) polymorphisms were carried out using PCR-RFLP. The results of CDKAL1 gene rs7754840 SNP in T2DM and control persons under the co-dominant model revealed that T2DM patients with the heterozygous genotype (GC) (OR = 1.93, CI 95%; 1.32–2.34, P