Demonstration of a mitochondrial DNA-compatible workflow for genetically variant peptide identification from human hair samples

Highlights•A mtDNA-compatible protein extraction workflow was developed for hair analysis. •mtDNA and genetically variant peptides (GVPs) were obtained from a hair sample. •Reproducibility of the new workflow was verified by a second laboratory. •GVPs were identified in all samples and putative GVP...

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Veröffentlicht in:	Forensic science international : genetics 2019-11, Vol.43, p.102148-102148, Article 102148
Hauptverfasser:	Catlin, LA, Chou, RM, Goecker, ZC, Mullins, LA, Silva, DSBSS, Spurbeck, RR, Parker, GJ, Bartling, CM
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult DNA Fingerprinting - methods DNA, Mitochondrial - genetics Female Genetically variant peptides Genome, Mitochondrial Genotyping Techniques Hair Hair - chemistry Haplotypes High-Throughput Nucleotide Sequencing Human identification Humans Male Mass Spectrometry Middle Aged Mitochondrial DNA Non-synonymous single nucleotide polymorphisms Pathology Peptides - analysis Peptides - genetics Polymerase Chain Reaction Polymorphism, Single Nucleotide Proteomics Sequence Analysis, DNA Workflow Young Adult
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Beschreibung
Zusammenfassung:	Highlights•A mtDNA-compatible protein extraction workflow was developed for hair analysis. •mtDNA and genetically variant peptides (GVPs) were obtained from a hair sample. •Reproducibility of the new workflow was verified by a second laboratory. •GVPs were identified in all samples and putative GVP profiles were obtained. •Viable mt-genome profiles were obtained using the combined workflow.
ISSN:	1872-4973 1878-0326
DOI:	10.1016/j.fsigen.2019.102148