Functional potato bioactive peptide intensifies Nrf2-dependent antioxidant defense against renal damage in hypertensive rats

[Display omitted] •Hypertension-associated renal damage is ameliorated by the bioactive peptide IF.•IF activates the antioxidant pathway via the upregulation of Nrf2 in hypertensive kidneys.•The bioactive peptide IF improves the survival of renal cells via the DJ-I and AKT axes.•IF might be an alternative therapy for reducing hypertension-associated renal damage. Hypertension, which is known as a silent killer, is the second leading cause of kidney failure worldwide. Elevated blood pressure causes approximately 7.6 million deaths, which account for ~13.5% of the total deaths and will continue to rise. High blood pressure is the prime risk factor associated with complications in major organs, including the heart, brain and kidney. High blood pressure accelerates oxidative stress and thereby causes organ dysfunction through the production of reactive oxygen species. In this study, we investigated the renal-protective effects of the bioactive peptide IF from alcalase potato protein hydrolysate in spontaneously hypertensive rat kidney. Sixteen-week-old spontaneously hypertensive rats were divided into three groups (n = 6), and Sixteen-week-old Wistar Kyoto rats (n = 6) served as the control group. The rats were administered IF and captopril via oral gavage for 8 weeks and then sacrificed, and their kidneys were harvested. The kidney sections from the rats treated with IF showed restoration of the structure of the glomerulus and Bowman’s capsule. The expression levels of Nrf2-mediated antioxidants were also increased, as confirmed by 4-hydroxynonenal immunohistochemical staining. The TUNEL assay revealed a significant reduction in the number of apoptotic cells in the IF-treated groups, which was consistent with the western blot results. Thus, the bioactive peptide IF exerts potential protective effects against hypertension-associated ROS-mediated renal damage via the Nrf2-dependent antioxidant pathway along the DJ-1 and AKT axes. Hence, we speculate that IF might have promising therapeutic effects on renal damage associated with hypertension.