Isolation of carbapenem-resistant Enterobacteriaceae harbouring NDM-1, 4, 5, OXA48 and KPC from river fish in Vietnam

The spread of potentially life-threatening carbapenem-resistant bacteria is a major threat to public health. This study aimed to determine the prevalence of carbapenem-resistant Enterobacteriaceae in fishery products in Vietnam. A total of 103 river fish were purchased from retail outlets and superm...

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Veröffentlicht in:Food control 2022-03, Vol.133, p.108594, Article 108594
Hauptverfasser: Nakayama, Tatsuya, Hoa, Tran Thi Tuyet, Huyen, Hong Mong, Yamaguchi, Takahiro, Jinnai, Michio, Minh, Doan Tran Nguyen, Hoang, Oanh Nguyen, Thi, Hien Le, Thanh, Phong Ngo, Hoang Hoai, Phuong, Nguyen Do, Phuc, Van, Chinh Dang, Kumeda, Yuko, Hase, Atsushi
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Sprache:eng
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Zusammenfassung:The spread of potentially life-threatening carbapenem-resistant bacteria is a major threat to public health. This study aimed to determine the prevalence of carbapenem-resistant Enterobacteriaceae in fishery products in Vietnam. A total of 103 river fish were purchased from retail outlets and supermarkets in Vietnam. The gut contents of fish were removed, mixed with buffered peptone water, and incubated for 24 h. The bacterial broth was spread onto CHROMagar™ ECC containing meropenem. Enterobacteriaceae were selected and antibiotic susceptibility testing was performed. Bacterial identification and genotyping were performed using 16S rRNA and multiplex PCR, respectively. Our results showed that 6.8% (7/103) of river fish were contaminated with carbapenem-resistant Escherichia coli harbouring NDM-1 or -5, and 8.7% (9/103) of the samples were contaminated with carbapenem-resistant Enterobacteriaceae harbouring NDM-1, -4, and OXA-48 genes. E. coli and Citrobacter freundii harbouring the NDM-1 encoding IncA/C plasmid were isolated from one fish sample; carbapenem-resistant E. coli harbouring CTX-M-15, NDM-5, and mcr-1 were also isolated. In conclusion, bacteria isolated from fishery products were found to carry the carbapenem resistance genes NDM, OXA-48, and KPC. As carbapenem-resistant bacteria are isolated from Vietnamese fishery foods at a rate of 6.8%, it is necessary to re-consider food hygiene rules. •6.8% river fish positive for carbapenem-resistant E. coli harbouring NDM-1 or -5.•Carbapenem-resistant Enterobacteriaceae harboured NDM-1,-4, -5, OXA-48, or KPC.•E. coli and C. freundii harbouring NDM-1 encoding IncA/C plasmid in one sample.•Not only E. coli but also other Enterobacteriaceae were resistant to carbapenems.
ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2021.108594