Generic and Shiga toxin-producing Escherichia coli (O157:H7) contamination of lettuce and radish microgreens grown in peat moss and perlite

Pathogens can be transferred to microgreens from seeds, irrigation water and growth media. The purpose of this study was to evaluate the contamination of Shiga toxin-producing Escherichia coli (STEC O157:H7) and generic E. coli to lettuce and radish microgreens grown in spray and bottom irrigated peat moss and perlite. Lettuce and radish seeds were grown in nalidixic acid resistant E. coli strains inoculated peat moss and perlite. Populations of cells were enumerated on edible and inedible part of plants. Survival of E. coli strains were also examined in growth media for 28 days. Type of irrigation did not affect the population of cells transferred to edible part of plants (P > 0.05). Populations of E. coli contaminating the inedible part of plant were higher in perlite than those in peat moss (P