High-throughput method based on a novel thin-film microextraction coating for determining macrolides and lincosamides in honey

•ZIF-8@GO was synthesized and applied as thin-film microextraction coating.•Thin-film microextraction with UPLC-MS/MS for antibiotics detection in honey was constructed.•Automated preparation offers simultaneous extraction of up to 96 samples per run. A high-throughput method using a new ZIF-8@GO thin-film microextraction coating was established for determining macrolides and lincosamides in honey. The coating preparation parameters (ZIF-8@GO synthesis conditions, coating material proportions, dipping time) and analysis parameters (sample diluent solvent, adsorption and desorption conditions using the ZIF-8@GO coating) were optimized. The optimized parameters were: diluent solvent sodium carbonate/sodium bicarbonate buffer solution (pH 9), adsorption time 45 min, desorption time 5 min, desorption solvent 45:40:15 v/v/v methanol/acetonitrile/water. The extracted targets were determined by ultra-high performance liquid chromatography tandem mass spectrometry. The recoveries of 10 analytes were 67.5–107.2% and the detection and quantification limits were 0.1–0.4 and 0.4–1.4 μg/kg, respectively. The method could analyze 96 samples per run. The minimal manual time and effort is required since the bulk of the sample processing is fully automated. It was a useful and efficient method for monitoring drug residues and was successfully used to analyze real samples.