In vitro assessment of the mutagenic and genotoxic potential of a pure stilbene extract

Stilbenes are secondary metabolites of great interest produced by many plant species due to their important bioactive properties. These phytochemicals have become of increasing interest in the wine industry as a natural alternative to sulphur dioxide, which has been associated with human health risks. However, there is still little toxicological information on stilbenes and the results thus far have been contradictory. Considering the key role of genotoxicity in risk assessment and the need to offer safe products in the market, the aim of this study was to assess the mutagenic and genotoxic potential of a stilbene extract with 99% purity (ST-99 extract). A complete series of different in vitro tests (Ames test, micronucleus (MN) test, and standard and enzyme-modified comet assays) was performed before its use as a preservative in wines. The ST-99 extract induces a significant increase of binucleated cells with micronuclei only in presence of the metabolic fraction S9 at the highest concentration assayed. Neither the Ames test nor the comet assay revealed the extract's genotoxic potential. Further studies are necessary, including in vivo assays, to ensure consumer safety before it can be used. •ST-99 extract protects and repairs DNA oxidative damage in Caco-2 and HepG2 cells.•MN test in presence of S9 indicated a weak genotoxic potential of ST-99 extract.•ST-99 showed negative genotoxicity results by Ames test and comet assay.•An individual toxicological evaluation of stilbenes extracts should be carried out.