Diet with Low Molecular Weight Chitosan exerts neuromodulation in Rotenone induced Drosophila model of Parkinson's disease

Rotenone (ROT) is well known pesticide been used in agriculture due to its low cost and rapid action. This has also been widely used to establish Parkinson's disease (PD) models in rodents. Here we have evaluated the role of well established potent molecule ‘Low Molecular Weight Chitosan (LMWC)’ against ROT induced neurotoxicity in Drosophila melanogaster. Male adult flies (8–10 days) were induced for neurotoxicity using ROT (500 μM) and treated with LMWC (5 & 10 mg/mL basal media) during 7 days experiment. Subsequently, neurodegenerative and behavioural parameters were assessed. As a result, flies exposed to ROT alone showed decreased locomotor behaviour in negative geotaxis and open-field studies and had a higher incidence of mortality compared to control group. These effects were accompanied by decrease in dopamine level, ETC complexes and increase in reactive oxygen species (ROS) production and cholinergic activities in head and body of the flies, suggesting ROT was able to induce oxidative stress. Co-exposure of LMWC reversed the ROT induced locomotor impairment, exploratory deficits and changes the biochemical parameters to normal level and increased the survival rate to 16 days. Thus, we suggested LMWC may be considered as better supplement in the prevention and management of Parkinson's disease. [Display omitted] •Rotenone (ROT) induces mitochondrial dysfunction in Drosophila melanogaster.•ROT increases AChE activity and diminish dopamine levels in the head and body of flies.•LMWC alleviated locomotors deficits caused by ROT in flies.•LMWC enhanced the activity levels of ETC enzymes and restored dopamine levels.•Dietary enriched LMWC prevents neurotoxic effects induced by ROT in flies.