Carvacrol inhibits cytochrome P450 and protects against binge alcohol-induced liver toxicity

Alcoholism is a serious addiction that can lead to various health complications such as liver fibrosis, steatosis, and cirrhosis. Carvacrol is present in many plant-based essential oils and used as a preservative in the food industry. In this study, we have investigated the hepatoprotective role of carvacrol against ethanol-induced liver toxicity in mice. To determine the effect of carvacrol on liver injury parameters, 5 doses of 50% ethanol (10 mL/kg body weight) were orally administered every 12 h for inducing the hepatotoxicity in experimental mice. Interestingly, carvacrol pre-treatment (50 and 100 mg/kg) reversed the ethanol-induced effects on liver function, antioxidant markers, matrix metalloproteinases activities, and histological changes. Moreover, carvacrol binds to the active pocket of cytochrome P450 (Cyt P450) and inhibits its expression. Thus, our finding suggests carvacrol can be used as an adjuvant for the amelioration of alcohol-induced hepatotoxicity. [Display omitted] •This study reports hepatoprotective potential of carvacrol.•Carvacrol abrogates MMP's level compared to alcohol intoxication group.•Carvacrol abolishes cytochrome P450 expression vs. alcohol intoxication group.•Carvacrol reduces inflammatory markers compared to alcohol intoxication group.•Carvacrol maintains autophagy in comparison to alcohol intoxication group.