VvERF17 mediates chlorophyll degradation by transcriptional activation of chlorophyll catabolic genes in grape berry skin

•VvERF17 was an ethylene response factor, the expression of which was strongly correlates with chlorophyll degradation.•Transgenic expression of VvERF17 modulated chlorophyll content and related genes’ expression.•VvERF17 could activate the expression of major chlorophyll catabolic genes by directly binding to the ERF binding sites in their promoters.•VvERF17 mediates chlorophyll degradation by transcriptional activation of NOL, PPH, PAO, and RCCR. Chlorophylls in the skin contribute to grape (Vitis vinifera) berry color and quality. Degradation of chlorophyll occurs during berry development in grape, but less is known about the molecular mechanisms of this process. Here, we characterized an ethylene response factor, VvERF17, as a putative regulator of chlorophyll degradation in grape berry. VvERF17 expression was strongly correlates with development-induced and ETH-triggered chlorophyll degradation. Transient overexpression of VvERF17 in tobacco leaves, tomato fruits, and grape leaves led to color break and chlorophyll degradation, which were resulted from the upregulated expression of chlorophyll catabolic genes (CCGs), including Chlorophyll-b-reductase (NOL), pheophytinase (PPH), pheophorbide a oxygenase (PAO), and red Chlorophyll catabolite reductase (RCCR). Inversely, silencing of VvERF17 in grape leaves partially suppressed the expression of CCGs’ expression. Major CCGs’ expression was closely related to chlorophyll degradation and VvERF17 expression. It was further demonstrated that VvERF17 could activate the expression of major CCGs by directly binding to the ERF binding sites in their promoters. We conclude that VvERF17 mediates chlorophyll degradation by transcriptional activation of NOL, PPH, PAO, and RCCR. These findings elucidate the regulation mechanisms of chlorophyll degradation in grape and enrich our understanding of grape berry color formation.