Synthesis and evaluation of 2,5-furan, 2,5-thiophene and 3,4-thiophene-based derivatives as CXCR4 inhibitors

The interaction between G-Protein coupled receptor CXCR4 and its natural ligand CXCL12 has been linked to inflammation experienced by patients with Irritable Bowel Disease (IBD). Blocking this interaction could potentially reduce inflammatory symptoms in IBD patients. In this work, several thiophene...

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Veröffentlicht in:European journal of medicinal chemistry 2019-11, Vol.181, p.111562, Article 111562
Hauptverfasser: Gaines, Theresa, Garcia, Francisco, Virani, Saniya, Liang, Zhongxing, Yoon, Younghyoun, Oum, Yoon Hyeun, Shim, Hyunsuk, Mooring, Suazette Reid
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Sprache:eng
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Zusammenfassung:The interaction between G-Protein coupled receptor CXCR4 and its natural ligand CXCL12 has been linked to inflammation experienced by patients with Irritable Bowel Disease (IBD). Blocking this interaction could potentially reduce inflammatory symptoms in IBD patients. In this work, several thiophene-based and furan-based compounds modeled after AMD3100 and WZ811—two known antagonists that interrupt the CXCR4-CXCL12 interaction—were synthesized and analyzed. Fifteen hit compounds were identified; these compounds exhibited effective concentrations (EC) lower than 1000 nM (AMD3100) and inhibited invasion of metastatic cells by at least 45%. Selected compounds (2d, 2j, 8a) that inhibited metastatic invasion at a higher rate than WZ811 (62%) were submitted for a carrageenan inflammation test, where both 8a and 2j reduced inflammation in the same range as WZ811 (40%) but did not reduce inflammation more than 40%. Select compounds were also modeled in silico to show key residue interactions. These preliminary results with furan-based and thiophene-based analogues contribute to the new class on heterocyclic aromatic-based CXCR4 antagonists. [Display omitted] •Fifty-six potential furan and thiophene based CXCR4/CXCL12 modulators synthesized.•Sixteen hit compounds inhibited metastasis of breast cancer cells by 50% or more.•Two hit compounds reduced carrageenan induced inflammation by 30%.•In silico analysis suggests hit compounds interact with key CXCR4/CXCL12 residues.
ISSN:0223-5234
1768-3254
DOI:10.1016/j.ejmech.2019.111562