Graphene oxide enters the rice roots and disturbs the endophytic bacterial communities

The environmental release of graphene oxide (GO) will certainly induce the GO exposure to plants. To date, the influence of GO on the intracellular structures and the endophytic bacterial ecology of plants have been rarely reported. In the present study, the rice seedlings were exposed to GO (5 mg/L) under hydroponic condition for fifteen days with periodic stir. The cellular structures damage, GO deposition and oxidative stress were found in rice root after GO exposure. A Illumina analysis based on the bacterial 16 S rRNA gene showed that the richness, evenness and diversity of endophytic bacterial communities of rice root decreased due to GO exposure. The relative abundance of beneficial endophytic bacterial populations decreased after GO exposure. Out of potential phenotypes predicted by BugBase, the relative abundance of Gram negative, stress-tolerant and biofilm-forming phenotypes, presented an increase trend after GO exposure. Grapheme oxide enters the rice roots and disturbs the endophytic bacterial communities. [Display omitted] •GO exposure induced GO depositions, cellular damage and oxidative stress in rice roots under hydroponic condition.•The relative abundance of many endophytic bacterial communities in rice roots decreased due to GO exposure.•The relative abundance of Gram negative, stress-tolerant and biofilm-forming phenotypes were predicted to increase by BugBase.