The effects of temperature on pathogenicity of entomopathogenic fungi for controlling larval populations of the European grapevine moth (Lobesia botrana) (Lepidoptera: Tortricidae)

The European grapevine moth (Lobesia botrana) is currently the most damaging pest in many viticultural regions of South and Central Europe and is highly responsible for the development of secondary infections by Botrytis cinerea to both table and wine grapes. Entomopathogenic fungi (EPF) are considered as substantial biological control agents of insect pests. However, they are able to infect their hosts only under a narrow spectrum of environmental conditions. In this study, the effects of temperature on pathogenicity of EPF for the control of L. botrana are reported. Among a collection of 33 EPF isolates, six virulent isolates were selected for the experiments. The bioassays took place at controlled conditions, under the temperature regimes of 20, 22.5, 25, 27.5, 30, and 32.5 °C. A conidial concentration of 1 × 108 conidia/ml was prepared for each isolate, and fungi were applied by spraying them on third-instar larvae (L3) of L. botrana. Both factors, fungal isolate and temperature, significantly affected mortality of L. botrana. Most fungal isolates exhibited their highest larval mortality rates seven days post-treatment at the temperatures of 25 and 27.5 °C. Some isolates caused considerably high larval mortality that exceeded 90% under specific temperature conditions. Overall, EPF showed great potential as bio-control agents for controlling larval populations of L. botrana. [Display omitted] •Entomogenous fungi showed great potential as biocontrol agents of Lobesia botrana.•Temperature significantly influenced all fungi on larval mortality of L. botrana.•An isolate from a cool region (Isaria fumosorosea EBAC01) was the most virulent.•Most fungal isolates demonstrated their highest efficacy at 25 and 27.5 °C.