SoybeanTracer: An In-Field scene property-based framework for high-throughput soybean canopy coverage extraction and evaluation

•SoybeanTracer achieved accurate extraction and evaluation of soybean canopy coverage.•SoybeanTracer framework was optimized by introducing in-field scene properties.•SCSNet significantly improved the result of soybean canopy coverage extraction.•Rationality of evaluation system in SoybeanTracer was validated on 196 plots. Soybean is a crucial plant-based protein and vegetable oil source for the global population and a significant cereal-oil dual-purpose crop. Extracting soybean canopy coverage, subsequently implementing evaluation in a low-cost, high-throughput and accurate manner, is highly valuable for soybean breeding and yield increase. Although the utilization of RGB remote sensing images for soybean analysis offers distinct advantages in terms of its cost effectiveness, there are still issues to be addressed. Specifically, the in-field scene properties of soybean canopy data have rarely been developed and there is a gap in automatic evaluation of soybean canopy coverage. For purpose of tackling these pending issues, this study proposed SoybeanTracer, a novel framework designed to achieve accurate soybean canopy coverage extraction and enable specialized evaluation with low cost and high throughput. The proposed framework comprised two components: Soybean Canopy Segmentation Network (SCSNet) and an agronomic soybean coverage evaluation system. Specifically, SCSNet employed a feature extraction network integrating the homogeneity within plot regions and soybean distribution characteristics. To further address the segmentation challenge of soybean plants with sparse growth posed by genotypes, a soybean multiscale feature fusion module was designed. As a result, SCSNet achieved a mean intersection over union (mIoU) and mean pixel accuracy (mPA) of 89.39% and 94.42%, respectively, on soybean canopy segmentation. Therefore, accurate and low-cost extraction of soybean canopy coverage was achieved through the use of RGB remote sensing data. Furthermore, the soybean canopy coverage evaluation system proposed in this study encompassed five levels, characterized by wider spacing at the two ends and narrower spacing in the middle. This agronomic evaluation system had been effectively implemented on 196 plots. The variance in the pairwise levels of the coverage evaluation results demonstrated the robustness of the evaluation system. SoybeanTracer could indeed help to extract soybean canopy coverage and provide valuable reference for implementing soybean genotype