Capillary electrophoresis Western blot using inkjet transfer to membrane

•Western blotting is performed from nanogram scale samples.•Protein samples are separated and captured on a membrane in 15 minutes.•Fluorescent antibodies are used to simultaneously detect ladder and analyte. Traditional Western blots are commonly used to separate and assay proteins; however, they have limitations including a long, cumbersome process and large sample requirements. Here, we describe a system for Western blotting where capillary gel electrophoresis is used to separate sodium dodecyl sulfate-protein complexes. The capillary outlet is threaded into a piezoelectric inkjetting head that deposits the separated proteins in a quasi-continuous stream of