Parallel chiral sub/supercritical fluid chromatography screening as a framework for accelerated purification of pharmaceutical targets

•A parallel multicolumn SFC workflow is introduced to speed up method development.•Automated chiral screening displays a high success rate.•A rapid deployment of enantioseparations for analysis and purification is achieved.•Fast-paced method development via parallel screening with minimized manual intervention. Chiral sub/supercritical fluid chromatography (SFC) has established itself as one of the preferred techniques for enantioseparations at both analytical and preparative scale. Herein, we introduce a parallel multicolumn SFC screening for automated chiral method development in fast-paced settings. The practicality and speed advantages of this approach are illustrated with parallel screening of a diverse set of chiral molecules across ten columns with five different organic modifiers/CO2 based eluents enabling rapid identification of suitable enantioseparation conditions for accelerated purification of pharmaceutical targets. Rapid delivery turnarounds of pure enantiomers of less than 1 h from screening to target isolation are demonstrated illustrating the power of this approach.