Theoretical study of twin-column recycling chromatography with a solvent-gradient for preparative binary separations

•Separation of twin-column recycling chromatography with a solvent gradient was simulated.•Solvent gradient induced a band compression effect.•The operation was simple without the need to determine adsorption isotherms in advance.•>Band broadening was counterbalanced by band compression. Twin-column recycling chromatography with a solvent gradient (TCRC-SG) was investigated with the equilibrium-dispersive chromatography model. The solvent gradient caused by constant addition of a modifier between the two columns created a band compression effect to counterbalance band broadening, so that the target component band neither broadened nor shrunk. Meanwhile, band compression accelerated the separation but prevented excessive separation. Increasing the volume fraction of weak solvent in the modifier and reducing the modifier flowrate enhanced band compression and improved the separation. The effect of column efficiency (number of theoretical plates: 500–1500) on the separation was not significant. According to the separation behavior, a simple operation scheme is proposed to automatically control column switching without needing to determine the adsorption isotherm and designing operating conditions in advance. In comparison with simulated moving bed, TCRC-SG had a higher feed throughput, but consumed more solvent. The results showed that TCRC-SG is favorable for preparative separation.