Mutual effect between electrochemically active bacteria (EAB) and azo dye in bio-electrochemical system (BES)

Herein, the mutual effect between azo dye and the performance of electrochemically active bacteria (EAB) is investigated in detail, which is crucial to understand and control the bio-electrochemical systems (BESs) operation for azo dye containing wastewater treatment. EAB is enriched at controlled potential of −0.2 V vs Ag/AgCl in single-chamber BESs. Over 95% azo dye (alizarin yellow R (AYR)) was decolorized regardless of the initial AYR concentration ranging from 30 to 120 mg/L within 24 h. The fastest decolorization rate was obtained at AYR initial concentration of 70 mg/L, which was 4.25 times greater in the closed circuit BESs than that in the open circuit one. 16S rRNA gene based microbial community analysis showed that Geobacter was dominant in EAB with relative abundance increased from 77.98% (0 mg/L AYR) to 92.22% (70 mg/L AYR), indicating that azo dye selectively boosts the growth of exoelectrogens in electrode biofilm communities. Under electricity stimulation, extracellular process can be mutually conducted by azo dye compounds, which is favorable for accelerating reaction rate and avoiding of significant toxic effect on EAB. [Display omitted] •Mutual effect between EAB and azo dye was comprehensively studied.•EAB with electricity stimulation exhibits high activity for AYR decolorization.•EAB dominant by Geobacter decolorized AYR via extracellular electron transfer.•Azo dye stimulates the growth of exoelectrogens (Geobacter) in a mixed EAB.