Knocking-out ARO80 promotes the intracellular ROS accumulation through weakening MAPK pathway of Saccharomyces cerevisiae

Through knocking-out of ARO80 (i.e., the key regulation gene for QS effect) from S. cerevisiae S288c, ΔARO80 strain was constructed to clarify the effect of QS on ROS related metabolisms and oxidative tolerance of yeast cells. Results presented here would help to understand the relationship between QS effect and oxidative and antioxidative metabolisms of S. cerevisiae. [Display omitted] •ARO80 deletion promoted ROS content but decreased oxidative tolerance of yeast cells.•QSMs reversed changes of ROS content and oxidative tolerance in ΔARO80 strain.•QS could regulate the oxidative and antioxidative metabolisms of S. cerevisiae.•RCK1 played role in QS associated regulation through weakening MAPK pathway. Previous works suggested a relationship between quorum sensing (QS) and reactive oxygen species (ROS)-related metabolisms in Saccharomyces cerevisiae, where ARO80 has been identified as the key gene regulating the QS. To clarify the effect of QS on ROS-related metabolisms and oxidative tolerance in yeast cells, we generated an ARO80 knockout (ΔARO80) strain of S. cerevisiae S288C. Deletion of ARO80 promoted ROS accumulation but decreased the oxidative tolerance of S. cerevisiae, while QS molecules (QSMs) reversed such changes. Transcriptomics analysis indicated 79 up-regulated and 55 down-regulated genes in the ΔARO80 strain compared to the wild-type strain. Based on our transcriptomics analysis and previous findings, the RCK1 was screened out as a target gene to be clarified. The ΔRCK1 strain exhibited higher ROS content but showed lower oxidative tolerance than the wild-type strain, suggesting a function in ARO80 deletion-associated regulation on ROS-related metabolism of S. cerevisiae by regulating the MAPK pathway.