Flexible and sustainable printed sensor strips for on-site, fast decentralized self-testing of urinary biomarkers integrated with a portable wireless analyzer

•Portable and user-friendly system for biomarkers monitoring toward health status diagnosis.•Low-cost sustainable devices for fast and sensitive decentralized measurement.•Noninvasive and on-site self-testing of urinary biochemical markers levels.•Good correlation between flexible, sustainable sensors and standard method.•Printed sensors on degradable PLA bio-plastic substrates/support from renewable resources. Flexible and sustainable electronic devices may fulfill the requirements of Sustainable Development Goals (SDGs) contained in Agenda 2030 from United Nations, however, the use of degradable and eco-friendly devices for rapid, selective and on-site decentralized monitoring of patient health status with portable systems toward diagnosis, fast decision and medical interventions is relatively new. Herein, we introduce flexible and sustainable substrates/support of degradable bio-based PLA films from renewable resources produced by casting method as a sustainable alternative to conventional petroleum-based plastics toward portable electronic devices and sensing applications. The flexible devices containing full electrochemical sensing system were built with screen-printing process on PLA sustainable substrates for on-site, rapid decentralized monitoring of molecular biomarkers in non-invasive sample. Reliable urinary dopamine and uric acid analysis with limit of detections of 0.138 µM and 0.595 µM could be performed with low-cost sustainable devices (