Enzyme-bimetallic hybrid catalyst for one-pot chemoenzymatic reactions

[Display omitted] •A facile and general method to synthesis enzyme-bimetallic hybrid catalyst was constructed.•With the confinement of enzyme aggregates, ultrafine bimetallic nanoclusters were synthesized and highly dispersed on enzymes.•The bimetallic nanoalloy showed excellent activity because of the strong bimetallic synergistic effect.•The highly active enzyme-bimetallic hybrid catalyst rendered the one-pot chemoenzymatic reaction to perform under mild conditions. To overcome the intrinsic incompatibility between enzyme and chemical catalysts in one-pot chemoenzymatic cascade reactions, an enzyme-bimetallic hybrid catalyst was constructed by in situ reduction of ultrafine PdCu naonoclusters on the cross-linked lipase aggregates. The PdCu nanoalloy showed excellent activity in the Sonogashira coupling reaction because of the strong bimetallic synergistic effect between Pd and Cu within PdCu/CALB CLEAs. The activity of 3.4Pd1.7Cu/CALB CLEAs was 2.6-fold of Pd/CALB CLEAs in the synthesis of (R)-N-[1-(4-(phenylethynyl)phenyl)ethyl] acetamide. The highly active PdCu/CALB CLEAs rendered the one-pot chemoenzymatic reaction to perform at a lower temperature.