Continuous production of a chiral amine in a packed bed reactor with co-immobilized amine dehydrogenase and formate dehydrogenase

Continuous production of a chiral amine in a packed bed reactor with co-immobilized amine dehydrogenase and formate dehydrogenase

•IMAC resin immobilization yielded 6.5 d half life and ≤443 g/(L∙d) productivity.•Overall reaction rate is limited by excess enzyme over cofactor but not mass transfer.•As [S] 

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Veröffentlicht in:Chemical engineering journal (Lausanne, Switzerland : 1996) Switzerland : 1996), 2021-03, Vol.407, p.127065, Article 127065
Hauptverfasser: Franklin, Robert D., Whitley, Joshua A., Caparco, Adam A., Bommarius, Bettina R., Champion, Julie A., Bommarius, Andreas S.
Format: Artikel
Sprache:eng
Schlagworte:
Amine dehydrogenase
Continuous flow
Michaelis-Menten kinetics
Packed-bed reactor
Reactor design
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Zusammenfassung:•IMAC resin immobilization yielded 6.5 d half life and ≤443 g/(L∙d) productivity.•Overall reaction rate is limited by excess enzyme over cofactor but not mass transfer.•As [S] 
ISSN:1385-8947
1873-3212
DOI:10.1016/j.cej.2020.127065