Valorisation of cheese whey as substrate and inducer for recombinant protein production in E. coli HMS174(DE3)

Every year worldwide around 190 million tons of cheese whey are generated resulting in a huge environmental burden. We recently published a study where we showed that E. coli strain HMS174(DE3) can be cultivated using only lactose as C-source and inducer. Motivated by the results we investigated usi...

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Veröffentlicht in:Bioresource technology reports 2019-12, Vol.8, p.100340, Article 100340
Hauptverfasser: Hausjell, Johanna, Miltner, Martin, Herzig, Christopher, Limbeck, Andreas, Saracevic, Zdravka, Saracevic, Ernis, Weissensteiner, Julia, Molitor, Christian, Halbwirth, Heidi, Spadiut, Oliver
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Sprache:eng
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Zusammenfassung:Every year worldwide around 190 million tons of cheese whey are generated resulting in a huge environmental burden. We recently published a study where we showed that E. coli strain HMS174(DE3) can be cultivated using only lactose as C-source and inducer. Motivated by the results we investigated using a concentrated whey feed instead of the lactose feed. Spray drying whey and dissolving the powder allowed preparation of a 40-fold concentrated whey containing 91% lactose and 81% protein of the original whey. Cultivations using the concentrated whey feed instead of a defined lactose feed revealed 39% higher growth rates, 24% higher biomass yields and even higher specific product titers for the model enzymes, flavanone 3-hydroxylase and chalcone 3-hydroxylase. Our strategy simultaneously provides a cheap substrate for large-scale production of technical enzymes and an excellent opportunity for cheese whey valorization, reducing the biological burden resulting from whey wastewaters. [Display omitted] •40-fold whey concentration by spray drying and dissolving the obtained powder.•Concentrated whey as C-source and inducer for recombinant protein production.•Physiological parameters on the concentrated whey feed.•Higher specific productivity on the whey feed compared to a defined lactose feed.
ISSN:2589-014X
2589-014X
DOI:10.1016/j.biteb.2019.100340