An interdigitated microelectrode based aptasensor for real-time and ultratrace detection of four organophosphorus pesticides

With increasing industrialization of food production, residues of organophosphorus pesticides (OPs) are more frequently found in the environment including rivers, lakes and soils. Extended exposure to OPs, even at a level below 1 nM, may lead to liver and central nervous system damages in humans and animals, while existing detection methods are not sensitive enough to detect OPs at trace levels. We presented a simple-to-use aptasensor to rapidly detect broad-spectrum OPs with high sensitivity. DNA aptamer was modified on the surface of a micro interdigitated electrode chip, and AC electrokinetics was employed to accelerate the binding of OP molecules to the aptamer probe. The sensing strategy directly measured the interfacial capacitance whose change rate was adopted as a quantitative indicator of recognition events, with a sample to result detection time of 30 s. This aptasensor had a wide linear range of (fM ~ nM), and the detection limit reached (0.24–1.67) fM for four highly-toxic OPs, with good specificity. It still showed good activity after being stored in non-refrigerated environment for at least 14 days. This aptasensor as well as the detection method offer a promising solution for on-site and real-time sensitive OP detection. •A highly sensitive aptasensor is presented for on-site real-time detection of organophosphorus pesticides (OPs).•Simultaneous detection of profenofos, isocarbophos, omethoate and phorate is achieved, with similar responses.•The sensor reaches a limit of detection at fM level, a wide linear range from fM to nM, and a good selectivity.•This sensor is easy to prepare, store and operate. It is effective in detecting practical OPs in emulsifiable concentrate.