Functional enzyme analysis and metabolic regulation mechanism of the combined microflora LXB in the degradation of lignocellulose

Biodegradation of lignocellulosic biomass is a practical and effective method. A random combination of six isolates and two purchased strains was constructed to obtain LXB that exhibited a lignocellulose degradation rate of 36.4 % after 168 h. The Carbohydrate-Active enZYmes Database identified 1872 genes that were differentially expressed and involved in the degradation of lignocellulose in LXB. The cellulase- and hemicellulase- encoding genes were highly expressed, with the hemicellulase- encoding genes displaying the highest expression levels during the initial and peak phases of lignocellulose degradation. A considerable increase was observed in the expression of genes encoding versatile peroxidases and alcohol oxidases involved in lignin catabolism. LXB catalyses the breakdown of lignocellulose via six major pathways within the carbohydrate metabolic network. Catechol, discovered in the benzoate degradation pathway, is involved in lignin degradation. Furthermore, LXB can degrade harmful exogenous substances, thus contributing to the overall detoxification process. [Display omitted] •The degradation rate of lignocellulose in combined microflora LXB was 36.4 %.•A total of 1872 functionally annotated differentially expressed genes were identified.•LXB primarily decomposes lignocellulosic biomass through six metabolic pathways.•Harmful exogenous substances can be metabolized by LXB.