Self-assembled pH-responsive DNA nanosponges for targeted co-delivery of doxorubicin and capsaicin for colorectal cancer therapy
DNA nanostructures have been engineered into various delivery systems for anti-cancer chemotherapy. However, these nanostructures are hindered in their therapeutic applications due to their inability to co-deliver drugs, weak biostability, the large size of the delivery system, and the high cost of...
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Veröffentlicht in: | Biochemical engineering journal 2023-06, Vol.195, p.108926, Article 108926 |
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Sprache: | eng |
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Zusammenfassung: | DNA nanostructures have been engineered into various delivery systems for anti-cancer chemotherapy. However, these nanostructures are hindered in their therapeutic applications due to their inability to co-deliver drugs, weak biostability, the large size of the delivery system, and the high cost of production. In this study, we developed a pH-controlled-release delivery system to target co-deliver Dox and CAPs solely to CRC cells. RCA technology produced high quantities of DNA-NS, which were then condensed with PEI. PEI shrank the size of DNA-NS from 1617 nm to 342 nm. Our novel delivery system intercalated the two drugs with a high loading efficiency of 78% at a ratio Dox to CAPs ratio of 1.16:1, which released less than 20% of the drugs at pH 7.4. AS1141 functional sites on DNA-NS efficiently facilitated higher cellular update and aided the DNA-NS/PEI to bond selectively to SW480 cells via the surface-expressed nucleolin. Dox and CAPs combinations had DRI scores >1 and CI scores |
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ISSN: | 1369-703X 1873-295X |
DOI: | 10.1016/j.bej.2023.108926 |