Ag@Sidr honey nanocomposite: Chemical profiles, antioxidant and microbicide procurator

Green synthesis of nanoparticles is recently required due to its ecofriendly and feasibility to be sequentially applicable in biomedical purposes. The current work investigated the affinity of Sidr honey (SH) to form bioactive silver based-nanocomposite. SH was exploited as reducing agent for Ag+1 ions and capping agent for the reduced Ag0 to form Ag@SH nanocomposite. The Metabolomic (LC-LTQ-MS/MS, 1 and 2-D NMR and GNPS) analysis showed that SH contains flavonoids, terpenoids and sugars (which were responsible for the reduction of Ag+1 ions). The overall data confirmed the formation of spherical shaped Ag@SH nanocomposite with average size ranged in 2.1–78.7 nm. The bioactivity of the obtained Ag@SH nanocomposite was evaluated by testing the microbicide activity and scavenging of DPPH radicals. The Ag@SH nanocomposite showed comparable antimicrobial results with the standard, whereas, strong inhibitory activity was observed against St. aureus bacteria (24 mm), F. solani fungi (12–15 mm) &C. albicans (17 mm). Comparing to the reference of vitamin C, Ag@SH nanocomposite exhibited very good antioxidant activity with DPPH radical scavenging percent of 76.6%. •Sidr honey (SH) was successfully exploited to reduce Ag0 forming Ag@SH nanocomposite.•Metabolomic analysis declared that SH contains flavonoids, terpenoids and sugars.•Ag@SH nanocomposite was obtained with spherical shape and with size of 2.1–78.7 nm.•Strong pathogen inhibition was observed against St. aureus (24 mm) and C. albicans (17 mm).•Quite good antioxidant activity was recorded with DPPH radical scavenging of 76.6%.