Utilization of banana (Musa sp.) fronds extract as an alternative carbon source for poly(3-hydroxybutyrate) production by Cupriavidus necator H16

Replacement of substrate by utilizing renewable, sustainable, abundance, and low-costs carbon sources derived from agricultural waste enables the reduction of wastes in industry and simultaneously capacitate the production of value-added products. The total amount of sugar in the banana frond extract (BFE) was 42.7 g/L, which in part consist of 10.5 g/L fructose. The effect of various BFE concentration on the PHB production was determined by conducting the fermentation utilizing BFE ranging from 10% to 50% (v/v) at 30 °C, 200 rpm for 72 h of incubation. The fermentation with 40% (v/v) BFE resulted in the highest PHB content in biomass (32.1%) and PHB concentration (0.9 g/L). Furthermore, the production of PHB was also examined by comparing the fermentation with 40% (v/v) raw and sucrase enzyme pre-treated BFE, respectively. The fructose content in the enzyme pre-treated BFE after enzymatic hydrolysis was increased to 14.6 g/L. The fermentation with 40% (v/v) pre-treated BFE showed higher PHB content in biomass (37.4%) and PHB concentration (1.3 g/L) as compared to the 40% (v/v) original BFE. The result indicates that 40% (v/v) of enzyme pre-treated BFE can be utilized as an alternative renewable and sustainable carbon feedstocks for PHB production. •BFE can be utilized as an alternative sustainable carbon source for PHB production by C. necator H16.•Effect of various BFE concentration for 10, 20, 30, 40, and 50% (v/v) on the PHB production was examined.•Fermentation with 40% pre-treated BFE showed highest PHB content in biomass (37.4%) and concentration (1.3 g/L).