Isolation, characterization of Berberine from Berberis aristata DC for eradication of resistant Helicobacter pylori

H. pylori have gradually acquired resistance to the commonly used antibiotics because of their use in many parasitic and anaerobic infections, which leads to treatment failure of various gastric and duodenal diseases associated with H. pylori infection. The present research work aimed to isolate and characterize the bioactive compound from the methanol extract of stem of Berberis aristata DC. This is traditionally used for the treatment of dyspepsia, dysentery and diarrhea against antibiotic-resistant gastric pathogen H. pylori. The in vitro antimicrobial activity of Berberine an active isolated compound from methanol extract of stem of Berberis aristata DC against drug-resistant H. pylori strain isolated from North Indian GERD patients. The H. pylori strain was only collected from those, who were devoid of any kind of anti-H. Pylori therapy. The methodology was in determining the Minimum inhibition concentration (MIC) using the microdilution method and disk diffusion method. H. pylori isolate was included in this study. Berberine from methanol extract of stem of Berberis aristata DC showed its potency on H. pylori-infected isolated from GERD patients with a maximum inhibition at 0.000075 μg/ml. Prevalence of metronidazole resistance ranges between 50 and 90% in developing countries including India. The emergence of dual drug resistance was reported in various studies. This study suggests that Berberine an isolated compound from methanol extract of stem of Berberis aristata DC used commonly known as Daru Haldi potentially active for the treatment of drug-resistant H. pylori infection. Berberine from methanol extract of stem of Berberis aristata DC with a concentration of 0.000075 μg/ml shows a positive effect safely and effectively. [Display omitted]