Genome sequencing, analysis and functional annotation of Bacillus isolated from sick silkworms, Bombyx mori

[Display omitted] •A novel bacterium isolated from silkworms could cause bacterial sepsis.•The whole gene size was 581 901 0 bp, including a chromosome and 5 plasmids.•Genomic analysis by Pfam, KEGG, COG, Genomic mapping and Pathogenic system. Laos was a country with a developing sericulture industry, but a high incidence of bacterial diseases happened in silkworms. The bacteria isolated from diseased silkworms collected in Laos were studied. The results showed that the four bacteria strains were isolated, The BmLT was the large number of silkworms infected and may be the mainly bacteria, its whole genome of BmLT was sequenced, was composed of a circular chromosome and 5 plasmids, with a genome of 5819010 bp and published in NCBI (PRJNA1111294). Six scaffolds were spliced, including 1 chromosome and 5 plasmids, of which 4 were known plasmids; one was a novel and published in NCBI (NZ-CP053290.1). Pfam annotation has 7 protein structure domains, 4 COG classifications, 22 COG types, and 4079 genes. The percentage of genes with COG annotation in all genes was 70.16 %. The GO and KEGG annotation analyses of annotated genes encompassed various metabolic and synthesis pathways, membrane transport, signal transduction, cell cycle, and disease-related pathways, involving 4392 genes, which accounted for 75.54 % of all genes. Genomic mapping analysis showed that it inclded seven Clusters of Orthologous Groups and 4th could encode inferred AbiEii toxins. Pathogenic system analysis showed, it included 541 virulence factor genes with promoting effects and 301 resistance genes, indicated that the bacteria had undergone pressure evolution from current sericulture and livestock production, as well as various antibiotics used by humans. PHI analysis showed it including 985 genes related to pathogen host, indicated,that it had undergone complex coevolution with silkworms or other hosts.