Transgenerational transfer of the microbiome is altered by ocean acidification in oyster larvae

Ocean acidification will affect marine molluscs, however, transgenerational plasticity (TGP) can ameliorate some effects. Marine molluscs acquire members of their microbiome via the egg, yet we know little about how the microbiome can be influenced by transgenerational exposure to ocean acidification. We exposed adult Sydney Rock oysters (Saccostrea glomerata) from four genotypes to elevated and ambient PCO2 for nine weeks. Larvae were then raised in the same ambient and elevated PCO2 conditions. The relative abundance of bacteria in eggs and larvae were characterised using 16S RNA amplicon sequencing. Parental exposure to elevated PCO2 significantly altered the bacterial community composition of both eggs and larvae, but this was dependent on genotype. Parental exposure to elevated PCO2 caused five core Rhodobacteraceae ASVs to increase in relative abundance, and three Rhodobacteraceae ASVs to decrease in relative abundance. These findings show transfer of maternal microbiomes to larvae is altered by exposure to ocean acidification and this may play a role in TGP. •Larval oysters inherit part of their microbiome from their parents.•Exposing adult oysters (Saccostrea glomerata) to ocean acidification altered the microbiome offspring.•These results differed among different genotypes of oysters.•Ocean acidification can have transgenerational consequences for the microbiome of oysters.