Effects of family and promoter on growth performance of ccGH cDNA transgenic channel catfish, Ictalurus punctatus, grown in a trough culture system

Constructs bearing growth hormone (GH) cDNA from channel catfish, Ictalurus punctatus, driven by the ocean pout, Zoarces americanus, antifreeze protein promoter (opAFP), or by the rainbow trout, Oncorhynchus mykiss, metallothionein promoter (rtMT) were transferred to channel catfish via electroporation. Transgenic P1 individuals were mated to produce F1 individuals that exhibited enhanced growth rate. The inheritance of the transgene by the F1 generation was 10% to 36%. The mean body sizes of F1 transgenic fish were larger than their non-transgenic full-siblings (P