Three generations of prawns without the Z chromosome: Viable WW Macrobrachium rosenbergii all-female populations in polyculture with Oreochromis niloticus

•This study presents the first field study of WW all-female M. rosenbergii populations produced by a novel biotechnology.•We show that this population can be sustained for consecutive generations with no visible ill-effects.•WW all-female population has both ecological and economic advantages and is suitable for polyculture with fish.